Journal: Veterinary Research

Article Title: Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells

doi: 10.1186/s13567-016-0328-y

Figure Lengend Snippet: Summary of the differentially expressed genes identified by analysis of the microarray results

Article Snippet: The second novel sequence investigated was identified as a novel transcript of TNFAIP3, which is not represented by any annotated probe-set on the Affymetrix bovine microarray.

Techniques: Microarray

Journal: Scientific Reports

Article Title: Combination of OipA, BabA, and SabA as candidate biomarkers for predicting Helicobacter pylori -related gastric cancer

doi: 10.1038/srep36442

Figure Lengend Snippet: KATO-III cells were incubated for 16 h with pooled DU-derived or GC-derived H. pylori (n = 5) at an MOI of 10, then were fixed and immunostained with FITC-labeled anti- H. pylori antibodies. The left panels show representative images and the right panel shows the quantified results expressed as the mean ± S.D. *p < 0.05. The data shown are representative of those obtained in three independent experiments.

Article Snippet: After 3 TBST washes, the array was assembled using a 3 × 7 well hybridization cassette (Arrayit Corporation, Sunnyvale, CA) and incubated sequentially in individual wells for 1 h at room temperature with serum (1:100 in 1% BSA in TBST), then, after 3 TBST washes, with Cy3-conjugated rabbit anti-human IgG + IgM + IgA antibodies (1:20000 in 1% BSA in TBST, Jackson Immunoresearch Laboratories).

Techniques: Incubation, Derivative Assay, Labeling

Journal: Scientific Reports

Article Title: Combination of OipA, BabA, and SabA as candidate biomarkers for predicting Helicobacter pylori -related gastric cancer

doi: 10.1038/srep36442

Figure Lengend Snippet: ( A ) Left, The selected antigens were purified and printed on a slide for fabrication of the GC-related protein microarray designed for a 3 × 7 well cassette. Middle , Individual serum samples from 7 normal individuals, 7 DU patients, and 7 GC patients were applied to individual wells. Right , The arrays were then incubated with Cy3-labeled anti-human IgG + IgM + IgA antibodies (green). The left panel shows a representative image of the probed GC-related array, while the panels on the right show a representative well, indicated in red on the left panel; the top panel shows the layout of the well and the bottom panel shows the result. Each antigen was printed in triplicate on the chip. Four concentrations of anti-human IgG + IgM + IgA antibodies (18.75–150 μg/ml) were used as positive controls, buffer was used as a negative control, and Cy3-labeled anti-mouse IgG was used as landmark. ( B ) Comparison of the seroreactivity of the samples from normal individuals, DU patients, or GC patients (n = 7 per group) with the three GC-related antigens, OipA, BabA, and SabA detected as the intensity of fluorescence of the Cy3-labeled anti-human IgG + IgM + IgA antibodies. *p < 0.05.

Article Snippet: After 3 TBST washes, the array was assembled using a 3 × 7 well hybridization cassette (Arrayit Corporation, Sunnyvale, CA) and incubated sequentially in individual wells for 1 h at room temperature with serum (1:100 in 1% BSA in TBST), then, after 3 TBST washes, with Cy3-conjugated rabbit anti-human IgG + IgM + IgA antibodies (1:20000 in 1% BSA in TBST, Jackson Immunoresearch Laboratories).

Techniques: Purification, Microarray, Incubation, Labeling, Negative Control, Comparison, Fluorescence

Journal: Journal of Veterinary Science

Article Title: Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis

doi: 10.4142/jvs.2018.19.1.79

Figure Lengend Snippet: Microarray analysis and validation of KDM4D mRNA expression in bMECs stimulated with Mycoplasma bovis ; bovine mammary epithelial cells (bMECs) were incubated with live M. bovis at an multiplicity of infection of 1,000 for 6 h. The data are expressed from three (microarray) or four (real-time polymerase chain reaction [PCR]) independent experiments. Significant difference ( * p <0.05) compared with unstimulated bMECs.

Article Snippet: We investigated gene expression in bMECs stimulated with or without live M. bovis by using an Agilent Bovine Gene Expression Microarray.

Techniques: Microarray, Expressing, Incubation, Infection, Real-time Polymerase Chain Reaction

Journal: STAR Protocols

Article Title: Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

doi: 10.1016/j.xpro.2024.103569

Figure Lengend Snippet:

Article Snippet: 10x BSA , Takara , 1166A.

Techniques: Virus, Recombinant, Transfection, Reverse Transcription, Amplified Luminescent Proximity Homogenous Assay, CCK-8 Assay, DNA Ligation, Microarray, Negative Control, Plasmid Preparation, Software, Spectrophotometry

Journal: STAR Protocols

Article Title: Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

doi: 10.1016/j.xpro.2024.103569

Figure Lengend Snippet: 10X annealing buffer

Article Snippet: 10x BSA , Takara , 1166A.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

doi: 10.1016/j.xpro.2024.103569

Figure Lengend Snippet:

Article Snippet: 10x BSA , Takara , 1166A.

Techniques:

Journal: STAR Protocols

Article Title: Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

doi: 10.1016/j.xpro.2024.103569

Figure Lengend Snippet:

Article Snippet: 10x BSA , Takara , 1166A.

Techniques: Plasmid Preparation

Journal: STAR Protocols

Article Title: Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

doi: 10.1016/j.xpro.2024.103569

Figure Lengend Snippet:

Article Snippet: 10x BSA , Takara , 1166A.

Techniques: